Sperm quality, egg size, fecundity and their relationships with fertilization rate of grass carp (Ctenopharyngodon idella)

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(2012-10-01)
Gametes were collected by abdominal massage from 15 anesthesized male and female grass carp. In collected sperm samples the following mean spermatological properties were determined: sperm volume 15.64 ± 5.39 mL, sperm motility 81.20 ± 7.61%, motility duration 62.06 ± 19.40s, density 17.88±5.48 x109 mL-1, total density 428.90±302.05 x109, and pH 7.25±0.81. Also egg size (mm) and fecundity (egg/fish) were determined as 1.04±0.028 and 417867 ± 36.274 respectively in female grass carp. The fertilization capacity of sperm was tested with the same egg pool. The insemination dosage was 2x105 spz/egg for each fertilization experiment. Fertilization rate was determined as mean 79.3±2.95% and positively correlated with sperm motility (r=0.932, p < 0.01), egg size (r=0.513, p>0.05) and fecundity (r=0.539, p>0.05).
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