Micropropagation of <i>Lantana camara</i> Through Axillary Shoots Proliferation

Abstract

<em>In vitro</em> budinduction and shoot regeneration of <em>Lantana camara </em>L. was investigated using axillary nodal explants. Multiple shoot buds were induced on Murashige and Skoog (MS) media supplemented with 0, 2, 4 and 8 mg/l of 6-benzylaminopurine (BAP) or thidiazuron (TDZ) for proliferation and 0, 0.25 and 0.5 mg/l indole-3 butyric acid (IBA) for rooting. After 4 weeks, a maximum number of shoot buds was achieved on MS media containing 8 mg/l BAP and most buds were resulted in 8 mg/l TDZ. The results showed that the maximum length of shoots, internode length and weight were observed in MS media without any growth regulators. Suitable concentrations of cytokinins significantly increased RNA, DNA and protein synthesis and shoot induction. Difference in concentrations of BAP can be under the influence of genetic factors and experimental condition. Very low concentrations of TDZ stimulated the proliferation of lateral shoots in woody plants. Receptor molecules of TDZ had high affinity with this growth regulator. In this research, the highest number of roots was induced in MS media with 0.25 mg/l IBA. IBA is one of the strong PGRs in culture medium for stimulating and increasing the number of roots. IBA increases internal IAA, which is necessary for normal growth of root meristems and final development of roots. One of the effects of auxins is elongation of cells, but root cells need lower auxin for growth because auxin induces ethylene which is a root growth inhibitory.