Coating of Iron Oxide Nanoparticles with Human and Bovine Serum Albumins: A Thermodynamic Approach

Abstract

In this research, the Magnetite nanoparticles (Fe304) were prepared by coprecipitation of Fe3+ and<br />FE solution in alkaline medium. Two kinds of surfactants, cetyl tri methyl ammonium bromide<br />(CTAB) and cetyl pyridinum chloride (CPC) were used in the synthesis. Fe304 Nanoparticles were<br />coated with human serum albumin (HSA) and bovine serum albumin (BSA). Characteristics of<br />coated magnetic nanoparticles and no coated were carried out using scanning electron microscopy,<br />X-ray diffraction and FT-IR spectroscopy. The interactions of colloidal iron oxide nanoparticles with<br />serum albumins, including BSA and EISA, were investigated by UV-Vis spectroscopy at process<br />different pH and temperatures. The thermodynamic parameters of coating were obtained from Van't<br />Hoof equation. The results revealed that both AM: and ASo of reactants positive values. These results<br />also demonstrate that p1-I can play an important role on adsorption of proteins on iron oxide<br />nanoparticles.