Investigating antibacterial activity of Satureja bachtiarica against coagulasenegative staphylococcus strains isolated from hospitalized patients in ICU,Tehran-Iran

Abstract

<br />Given the inappropriate use of antibiotics and prevalence of resistant bacteria, there is urgent<br />need for antibacterial drugs that have fewer side effects than antibiotics. Satureja bachtiarica<br />is a medicinal plant which had many uses in traditional medicine. In this research, the ethanol<br />extract of leave of Satureja bachtiarica is tested on coagulase-negative staphylococcus strains.<br />Materials and Methods<br />In this experimental study, after collecting and drying the plant extract, the ethanolic extract of<br />the plant was extracted by soxhelet method. Then, the antibiotic resistance pattern of coagulasenegative<br />staphylococcus strains to Cefoxitin, Tobramycin, Kanamycin, Amikacin, Gentamicin,<br />was assessed. To evaluate the antibacterial activity, the minimum inhibitory concentration was<br />assessed by using micro dilution broth method.<br />The results of this study showed that the antimicrobial effect of Satureja extract is concentration<br />dependent. The results of the antimicrobial activity of extracts, indicate that alcoholic extract<br />in all dilutions have an antimicrobial effect on the coagulase-negative staphylococcus strains.<br />The results show that coagulase-negative staphylococcus strains have highest resistance against<br />Cefoxitin (0.39 mg/ml) and highest sensitivity to Amikacin (0.035 μl/ml). However, in 200 and<br />100 mg/ml concentrations the extract has higher antibacterial effect.<br />According to the results of this study, ethanol extracts of leave of Satureja bachtiarica had<br />growth inhibitory effect on coagulase-negative staphylococcus strains. Therefore this plant has<br />the potential to be evaluated as an alternative or adjunct to antibiotics to treat coagulase-negative<br />staphylococcus infections.