The toxicity of zinc oxide on blood factors of Huso huso and Cyprinus carpio

Abstract

In this research, acute toxicity (LC) of zinc oxide nanoparticle on Beluga (Huso huso) and<br />common carp (Cyprinus carpio) was studied. The experiment was conducted using the<br />Organization for Economic Co-operation and Development (No. 202) method. For both<br />species, 96hLC10 was determined to be more than 200 mg/l. To assess the effects of zinc oxide<br />nanoparticles on blood factors, leukocyte index count (Lymphocyte, Monocytes, Neutrophils,<br />Eosinophil, and Basophils) was performed, and the results were analyzed using IBM SPSS<br />software version. According to the results and based on the ANOVA test, there is no significant<br />difference among mean values of leukocyte index responses (p>0.05), and one should note<br />that the amount of lymphocyte had decreased while that of neutrophils and monocytes had<br />increased. Furthermore, with regard to concentration levels, this decrease or increase has not<br />followed a systematic trend. In Beluga based on ANOVA test, there is a significant difference<br />between mean values of lymphocytes and neutrophils (p<0.05). Again, compared to control,<br />lymphocyte count increased while that of neutrophils and eosinophil decreased, and this too, did<br />not follow a systematic trend. Finally, concentration to 200 mg/l ZnO did not cause mortality in<br />Huso huso and Cyprinus carpio. Eventually, decline of body resistance against nano zinc oxide<br />was observed in these species through blood cells study.