Simultaneous determination of paracetamol, 4-Aminophenol, 4-Chloroacetanilid, Benzyl alcohol,Benzaldehyde and EDTA by HPLC methodin paracetamol injection ampoule

Abstract

Paracetamol that is known as acetaminophen have the most consume as an analgesic and antipyretic<br />drug in the world. That is formulated in single compound or mixture at many forms such as<br />tablets, syrups, suspensions and drops. The last form is intravenous injections. Paracetamol derived<br />from 4-minophenol which is synthesized by acylated the P-acetaminophenol and acetic anhydride.<br />4-aminophenol is the main impurity at manufacturing of paracetamol which could produce by<br />hydrolysis during storage or synthesis under normal conditions (temperature, pH, etc.). Also,<br />4-chloroacetanilid may be observed as an impurity in the raw material of paracetamol synthesis.<br />Benzyl alcohol is a preservative that used in Paracetamol for injection. It will be very important if<br />there are analytical techniques to measuring paracetamol and its degradation products accurately and<br />easily. Undoubtedly the most important and widely used, separation technique is chromatography.<br />There are several reports about separation and quantitative determination of paracetamol lonely or<br />simultaneous determination of paracetamol and 4-aminophenol. In this paper investigated simultaneous<br />determination of paracetamol, 4-aminophenol, 4-chloroacetanilid, benzyl alcohol, benzaldehyde,<br />and EDTA in paracetamol for injection ampoules by high performance liquid chromatography. By<br />changing the ratio of mixing methanol and acetonitrile as mobile phase at the wavelength of 215 nm<br />and pH=3 separation of all compounds were completely done.