USE OF ORGANIC SOLVENTS FOR THE ENHANCEMENT AND LIFETIME INCREASING OF LUMINESCENCE IN FLUORIMETRIC APPLICATION BY FLUORESCAMINE

Abstract

In this study, the fluorogenic efficiency of fluorescamine for spectrofluorimetric analysis of primary amines was investigated. Different mixtures of dichloromethane and other organic solvents were used for evaluation of fluorescence stability and intensity of fluorescamine derivative (Pyrrolinone). Solvents such as acetonitrile (ACN), tetrahydrofuran (THF) and dioxane increase stability and intensity of fluorescence, whereas methanol, acetic acid and dimethyl sulfoxide have a decreasing effect. Calibration graph equations in optimum solvent system were obtained for spectrofluorimetric determination of norepinephrine as a model compound in the range of 0.1 - 1 .O ppm in 10 minute intervals. Results obtained show a good correlation coefficient and excellent reproducibility (RSD% = 0.82, C = 0.5 ppm, n = 6). Using the first set of solvents, reproducibility of spectrofluorimetry is excellent (RSD% = 0.82).