Characterization and biocompatibility of hydroxyapatite nanoparticles extracted from fish bone

Abstract

In this study, hydroxyapatite (HAp) from fish bone was extracted by thermal calcination method, and then compared to chemical synthesized HAp. The samples were analyzed by Fourier Transform Infrared spectroscopy (FT-IR), X-Ray Diffraction analysis (XRD), Elemental analyze, Scanning Electron Microscopy, and cell tests. The FT-IR results showed well the presence of functional groups related to hydroxyapatite. The XRD results were in agreement with standard data. Size of the HAp Particle obtained about 300 and 50 nm for fish bone HAp and chemical synthesized samples, respectively. Good crystallinity and rod-like structures were showed for the fish bone HAp samples. The cell results with osteoblast-like cells showed well biocompatibility and proliferation of the cells on HAp nanoparticles. This study confirms that extracted fish bone is a promising biomaterial for bone regeneration.<br /> The cell results with osteoblast-like cells showed well biocompatibility and proliferation of the cells on HAp nanoparticles. This study confirms that extracted fish bone is a promising biomaterial for bone regeneration.