Protective Effects of Bacillus sp. MBIA2.40 and Gallipro on Growth Performance, Immune Status, Gut Morphology and Serum Biochemistry of Broiler Chickens Feeding by Aflatoxin B1

Abstract

A total of 540 one-day old male broilers Ross 308 were allocated to 6 treatments in a completely randomized designed with a 2×3 factorial arrangement (aflatoxin B1 (AFB1) and probiotics) with 6 replicates and 15 birds in each. Factors include aflatoxin B1 (control, 500 ppb) and probiotics (control, 108 cfu/mL Bacillus sp. MBIA2.40, 0.2 g Gallipro per kg). Results showed that adding 500 ppb AFB1 to broiler diets resulted in a significant decrease of body weight gain and feed intake (P < 0.05). The highest of feed conversion ratio was observed in AFB1 group (P < 0.05). Average body weight gain was significantly increased while feed conversion ratio decreased by Bacillus sp. MBIA2.40 and Gallipro compared with control group (P < 0.05). The supplementation of Bacillus sp. MBIA2.40 and Gallipro in contaminated diets relieved the negative effects of aflatoxin on performance. Increased serum aspartate amino transferase, alanine amino transferase, alkaline phosphatase and lactate dehydrogenase were observed in diet contaminated with AFB1 compared with other groups (P < 0.05). The lowest antibody production against Newcastle disease and sheep blood cells and also the lowest skin response to phytohemagglutinin were observed in the AFB1 group (P < 0.05). Villus height and width were significantly increased by probiotics diets without AFB1 in compared with others (P < 0.05). The crypt depth was significantly higher by birds fed AFB1 rather than that others (P < 0.05). Improving villus height, width and crypt depth by the diets containing probiotics + AFB1 were found as similar as the control diet (P > 0.05). In general, the results of this study showed that adding of Bacillus sp. MBIA2.40 and Gallipro in contaminated diets reduce negative effects of AFB1 and consequently these probiotics (especially Bacillus sp. MBIA2.40) have a protective effect against aflatoxicosis in broiler chickens.