Alleviation of Cisplatin-Induced Hepatotoxic Damage: the Synergistic Effect of Morin and Hesperidin against Oxidative Stress

Abstract

<strong>Background and objectives:</strong> A key aspect of cisplatin-induced hepatotoxicity is oxidative stress. The current study was conducted to show, for the first time, the restoring and synergistic effects of morin and hesperidin against oxidative stress in hepatotoxicity. <strong>Methods:</strong> Fourty-two Wistar albino rats were randomly divided into seven groups: group A (control), group B (morin), group C (hesperidin), group D (cisplatin), group E (cisplatin+morin), group F (cisplatin+hesperidin), group G (cisplatin+morin+hesperidin). Throughout ten consecutive days, morin and/or hesperidin were given to rats and cisplatin was injected as a single dose (7 mg/kg) on the 4^th day, and then the rats were sacrificed on the 11^th day. Liver tissue samples collected from the rats were used for the measurement of malondialdehyde, nitric oxide, glutathione levels as well as myeloperoxidase, catalase and superoxide dismutase activities. <strong>Results:</strong> Administration of cisplatin elevated the malondialdehyde and nitric oxide levels and also reduced the glutathione levels and catalase activity in the liver. However, in the morin and/or hesperidin groups, glutathione level and catalase activity were higher but malondialdehyde and nitric oxide levels and myeloperoxidase activity were lower than the cisplatin-induced group. <strong>Conclusion:</strong> Our results indicated that pretreatment with these flavonoids can be used as protective treatment for cisplatin-induced hepatotoxicity.