Functional Annotation of Two Hypothetical Proteins (LOC105974023 and LOC109219975), as Proteins Involved in Response to Salinity: An in silico Approach

Abstract

Through the exponential development in the specification of sequences and structures of proteins by genome sequencing and structural genomics approaches, there is a growing demand for valid bioinformatics methods to define these proteins function. In this study, our objective is to identify the function of unknown proteins from <em>UCB-1</em> <em>pistachio</em> rootstock and specify their classification using bioinformatics tools. In previous research, we recognized 5 <em>HPs</em> in proteomic profile of the University of California at Berkeley I <em>pistachio</em> rootstock leaf under salinity stress. The LOC105974023 and LOC109219975 proteins had 2.95 and 2.29-fold up-regulation under salinity stress, respectively. In this study, the probable function and characterization of these <em>HPs</em> were recognized using different statistical methods and programs. According to our analyses, these <em>HPs</em> have similarities with reverse transcriptase enzyme as well as helicase enzyme and some responsive proteins to salt stress. These observations suggest a close relationship between the overexpression of these enzymes and plant responses to salinity stress. These stress-responsive proteins could provide a novel plant defense strategy in response to salinity.