Correlation between Expression of hsa-miR-490-5p and NFAT5 in Peripheral Blood Mononuclear Cell Obtained from Breast Cancer Patients

Abstract

Breast cancer is a complex genetic disease that has an average annual incidence of two million people and the second leading cause of death among women all over the world. Micro-RNAs are consistently reported to regulate gene expression in all cancers. The present study, the correlation between the expression of hsa-miR-490-5p and nuclear factor of activated T-cells 5 (<em>NFAT5</em>) in breast cancer were investigated. <em>NFAT5</em> nuclear accumulation occurs regardless of Wnt/β-catenin activated signaling in a substantial portion of breast cancer. The analysis of prediction target and dual-luciferase reporter assays supported that hsa-miR-490-5p directly targeted <em>NFAT5</em> and suppressed the expression of <em>NFAT5</em>. In a cross-sectional comparative study, peripheral blood samples were collected from 30 subjects with breast cancer and 30 healthy individuals as a control group. Total mRNA was extracted from peripheral blood mononuclear cells (PBMCs) and cDNA was synthesized to study the <em>NFAT5</em> and hsa-miR-490-5p gene expression variations by real-time PCR. A significant decrease was observed in gene expression and sera concentration of <em>NFAT5</em>, hsa-miR-490-5p in PBMCs of breast cancer patients. The obtained results indicated that hsa-miR-490-5p acts as oncomir in serum by targeting <em>NFAT5</em> direct (p<0.05).