Numerical study of insulation structure characteristics and arrangement effects on cell trapping using alternative current insulating based dielectrophoresis

Abstract

Insulator-based dielectrophoresis is a recently developed technique in which insulating posts are used to produce non-uniformity in the electric field in a microchannel. This study presents the effects of insulating posts geometry and arrangement on the trapping efficiency of red blood cells in an alternating current- Insulator-based dielectrophoresis system. Microchannels containing square, circular and diamond-shaped posts with particles under the influence of positive dielectrophoresis force and fluid flow were considered. Finite element method was used to compute the velocity of the flow and electric field. The numerical method was verified by comparing the numerical results with experimental data. Two distinct criteria for examining particle trapping for distinct shapes and arrangements of insulating posts were introduced. Particle tracing simulation was implemented to observe particle trapping and compare the trapping performance of systems with distinct posts. As shown in the results for the system with circular and square posts, insulators should be narrowed to improve particle trapping, while diamond post should be widened to increase the trapping efficiency. In addition, the particle tracing results showed that microchannel with square posts is more efficient in particle trapping.