Effect of <i>Satureja khuzestanica</i> essential oil against fertility disorders induced by busulfan in female mice

Abstract

Busulfan is an alkylating agent affects ovarian follicles growth by oxidative stress induction. <em>Satureja khuzestanica</em> has antioxidant effects. The aim of this study was to examine whether <em>S. khuzestanica</em> essential oil (SKEO) exhibits protective effects on busulfan-induced ovarian failure. Eighty-four adult female mice were divided into six groups including dimethyl sulfoxide (control), SKEO 225.00 mg kg^-1 (orally), busulfan 3.00 mg kg^-1 (orally), busulfan 36.00 mg kg^-1 (intraperitoneally), busulfan 3.00 mg kg^-1 and SKEO and busulfan 36.00 mg kg^-1 and SKEO. After 28 days, the mice were euthanized and oocytes were removed for <em>in vitro</em> fertilization (IVF) rate evaluation. Oocyte quantity and quality, fertilization rate and pre-implantation embryo development were daily examined with a stereo microscope in a period of 120 hr. Serum levels of estradiol and progesterone were also evaluated. Busulfan caused significant decreases in oocyte number and quality, fertilization rate, pre-implantation embryo development and embryo quality. The SKEO significantly decreased the adverse effects of busulfan. The present study indicated that SKEO can protect female fertility potential against busulfan induced damages.