Electroanalytical Detection of Uric Acid on Blue HEGN Modified Glassy Carbon Electrode by Voltammetry

Abstract

An electroanalytical technique was advanced for the detection of uric acid (URI) relying on its oxidation behaviour. Using cyclic voltammetry (CV) techniques, the electrochemical performance and detection of URI were easily accomplished on poly (Blue HEGN) modified glassy carbon electrode (Po-BHEGN/GCE). The role of pH on anodic peak current and potential was examined. Phosphate buffer of 7.4 pH was opted for subsequent data analysis. Sweep rate studies were carried out and showed that electrode reaction was a diffusion-controlled process. A linear calibration curve was established in the URI concentration levels from 10-70 µM. The LOD and LOQ were estimated to be 0.94 and 2.91 µM, respectively. A simultaneous study of URI and dopamine (DA) revealed that well-separated peak at Po-BHEGN/GCE compare to GCE. To sum up, a straightforward and inexpensive sensor Po-BHEGN/GCE is built for the sensitive and focused detection of URI in samples.