Comparison of the Effect of Ginger and Nystatin Mouthwash on the Growth of Candida Albicans under in Vitro Conditions

Abstract

Background and Objective: Candidiasis is one of the most common diseases in the oral cavity. Due to the side effects of chemical mouthwashes, the use of antifungal herbs has increased significantly. Therefore, this study was performed to evaluate the effect of Ginger (Vi-one) mouthwash on the growth of Candida albicans under in vitro conditions. Methods: In this experimental in vitro study, 32 special solid culture media were prepared. 15 special solid culture media containing discs and wells impregnated with ginger mouthwash (Vi-one) and 15 special solid culture media with discs and wells of nystatin extract were prepared. Moreover, two blank sterile cultures with disks and wells containing no mouthwash or extract were prepared. These plates were placed in microaerophilic conditions with the presence of carbon dioxide and were transferred to the incubator at 37°C for 48 hours. After 48 hours, the zone of inhibition was determined using standard methods. Findings: Agar well diffusion method revealed that the zone of inhibition for ginger mouthwash equaled 23.6±0.33mm and for Nystatin equaled 28.2±1.2. Agar disc diffusion method revealed that zone of inhibition for ginger mouthwash equaled 18.3±1 and for Nystatin equaled 28.3±0.1 (p<0.0001). Conclusion: Based on the results of this study, it seems that ginger mouthwash (Vi-one) compared to nystatin extract can be effective against the growth of Candida albicans under in vitro conditions.