Facile fabrication and characterization of silver nanoparticles by sunn pest (Eurygaster integriceps puton) damaged wheat and evaluation of its antibacterial and cellular toxicity toward liver cancer cell lines

Abstract

Background & Aims: Silver nanoparticles (AgNPs) are among the most explored nanomaterials and are widely employed in medical, pharmaceutical, and bioengineering application. The aim of this study was to investigate the antibacterial and cell toxicity effects of fabricated AgNPs by green method at room temperature. Materials & Methods: In this work, AgNPs were fabricated by green method, which uses the seed extract of Triticum aestivum damaged by sunn pest (Eurygaster integriceps Puton) (SPDSPDWAgNPs) at room temperature. Powder X-ray Diffraction (PXRD) and Transmission Electron Microscopy (TEM) were used for characterization of synthesized nanoparticles. Disk diffusion method and MTT analysis were used for antibacterial and cell toxicity effects, respectively. Results: The synthesis of SPDSPDWAgNPs was relevant by color change of solution, turning from light-yellow to dark-brown, respectively. α-glycosidase, protease and α-amylase are some identified enzymes of salivary glands of sunn pest which can affect the synthesis of nanoparticles. The results showed that SPDSPDWAgNPs have spherical shapes and had a different range from 1 to 25 nm. Moreover, the biosynthesized SPDSPDWAgNPs have high antibacterial properties toward the most common inflectional bacteria. Furthermore, the cytotoxicity results show the fabricated SPDSPDWAgNPs prevent the growth of cancer cells. Conclusion: We concluded that this production manner of nanoparticles could be employed for large-scale synthesis of AgNPs, pharmaceutical, and antiseptic uses.